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TAXON

Plant Diversity and Resources

Journal of Systematics and Evolution

Volume 55 Issue 2, Pages 158C169.

Published Online: 21 Feb. 2017

DOI: 10.1111/jse.12238

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Fungal diversity and specificity in Cephalanthera damasonium and C. longifolia (Orchidaceae) mycorrhizas

Lorenzo Pecoraro1,2,3*, Laiqiang Huang2, Tancredi Caruso4, Silvia Perotto5, Mariangela Girlanda5, Lei Cai3, and Zhong-Jian Liu1

1Shenzhen Key Laboratory for Orchid Conservation and Utilization, The National Orchid Conservation Center of China and The Orchid Conservation and Research Center of Shenzhen, Shenzhen 518114, China

2Center for Biotechnology & BioMedicine and Division of Life & Health Sciences, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China

3State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

4School of Biological Sciences, Queen's University of Belfast, Belfast, BT7 1NN, Northern Ireland, UK

5Department of Life Sciences and Systems Biology, University of Turin, Via Verdi, 8-10124 Turin, Italy

Keywords: Ascomycetes; basidiomycetes; fungal symbionts; mycorrhizal specificity; Orchidaceae; orchid mycorrhiza

Abstract:

Orchids depend on mycorrhizal fungi for their nutrition, at least in the early stages of their growth and development, and in many cases throughout the life. In spite of the increasing number of studies describing fungal diversity in orchids, there is still more to be learnt about the identity of fungal partners and specificity in orchid mycorrhizal associations. We investigated the fungal communities associated with the roots of Cephalanthera damasonium (Mill.) Druce and C. longifolia (L.) Fritsch adult plants, using morphological methods and fungal internal transcribed spacer-DNA polymerase chain reaction amplification, cloning, and sequencing. A range of fungi belonging to Basidiomycota and Ascomycota was uncovered in the roots of the two investigated orchid species, showing a low degree of mycorrhizal specificity. At least 11 fungal taxa, including Cenococcum geophilum Fr., Ceratobasidium sp., Exophiala salmonis J. W. Carmich., Hymenogastraceae, and Sebacinaceae colonized C. damasonium roots, while approximately 9 fungal types, such as Bjerkandera adusta (Willd.) P. Karst., Phlebia acerina Peck, Sebacinaceae,Tetracladium sp., and Tomentella sp. associated with C. longifolia. Phylogenetic and statistical analyses indicated significant differences in the fungal communities associated with the two studied Cephalanthera species, as well as distinct mycorrhizal partners associated with each orchid plant. Our results strongly suggest that both C. damasonium and C. longifolia are generalist in their mycorrhizal associations.

 

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