Journal of Systematics and Evolution

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  • 收稿日期:2020-08-31 接受日期:2020-12-03

Targeted sequencing supports morphology and embryo features in resolving the classification of Cyperaceae tribe Fuireneae s.l.

Julian R. Starr1,*, Pedro Jiménez-Mejías2,3, Alexandre R. Zuntini4, Étienne Léveillé-Bourret5, Ilias Semmouri6, Muthama Muasya7, William J. Baker4, Grace E. Brewer4, Niroshini Epitawalage4, Isabel Fairlie4,8, Félix Forest4, Izai A.B. Sabino Kikuchi4,9, Lisa Pokorny4,10, and Isabel Larridon4,11   

  1. 1University of Ottawa, Department of Biology, Ottawa, Ontario, K1N 6N5, Canada

    2Departamento de Biología (Botánica), Facultad de Ciencias Biológicas, Universidad Autónoma de Madrid, C/ Darwin, 2, 28049 Madrid, Spain

    3Centro de Investigación en Biodiversidad y Cambio Global (CIBC-UAM), Universidad Autónoma de Madrid, 28049 Madrid, Spain

    4Royal Botanic Gardens, Kew, Richmond, Surrey, TW9 3AE, UK

    5Université de Montréal, Institut de recherche en biologie végétale (IRBV), Montréal, Québec, H1X 2B2, Canada

    6Ghent University, Faculty of Bioscience Engineering, Laboratory of Environmental Toxicology and Aquatic Ecology, 9000 Ghent, Belgium

    7Bolus Herbarium, Department of Biological Sciences, University of Cape Town, Private Bag X3, Rondebosch, 7701 Cape Town, South Africa

    8Department of Animal and Plant Sciences, University of Sheffield, Alfred Denny Building, Western Bank, Sheffield, S10 2TN, UK

    9Hortus botanicus Leiden, Universiteit Leiden, PO Box 9500, Leiden, 2300 RA, The Netherlands

    10Current address: Centre for Plant Biotechnology and Genomics (CBGP, UPM-INIA), 28223 Pozuelo de Alarcón (Madrid), Spain

    11Systematic and Evolutionary Botany Lab, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, 9000 Gent, Belgium

  • Received:2020-08-31 Accepted:2020-12-03

Abstract:

Molecular phylogenetic studies based on Sanger sequences have shown that Cyperaceae tribe Fuireneae s.l. is paraphyletic. However, taxonomic sampling in these studies has been poor, topologies have been inconsistent, and support for the backbone of trees has been weak. Moreover, uncertainty still surrounds the morphological limits of Schoenoplectiella, a genus of mainly small, amphicarpic annuals that was recently segregated from Schoenoplectus. Consequently, despite ample evidence from molecular analyses that Fuireneae s.l. might consist of two to four tribal lineages, no taxonomic changes have yet been made. Here, we use the Angiosperms353 enrichment panel for targeted sequencing in order to: (1) clarify the relationships of Fuireneae s.l. with the related tribes Abildgaardieae, Eleocharideae and Cypereae; (2) define the limits of Fuireneae s.s., and (3) test the monophyly of Fuireneae s.l. genera with emphasis on Schoenoplectus and Schoenoplectiella. Using more than a third of Fuireneae s.l. diversity, our phylogenomic analyses strongly support six genera and four major Fuireneae s.l. clades that we recognise as tribes: Bolboschoeneae stat.nov., Fuireneae s.s., Schoenoplecteae, and Pseudoschoeneae tr.nov. These results are consistent with morphological, micromorphological (nutlet epidermal cell shape), and embryo differences detected for each tribe. At the generic level, most sub-Saharan African perennials currently treated in Schoenoplectus are transferred to Schoenoplectiella. Our targeted sequencing results show that these species are nested in Schoenoplectiella, and their treatment here is consistent with micromorphological and embryo characters shared by all Schoenoplectiella species. Keys to recognised tribes and genera are provided.

Key words:

Angiosperms353, classification,  Cyperaceae,  Fuireneae,  targeted sequencing,  taxon limits, taxonomy