Abstract: Yunnan is extends across the subtropical and tropical zones, situated in the southern border area of southwestern China. This district is extremely rich in plants, and thus, it has been known as a “Kingdom of Plants”. However, up to the present our knowledge of the Charophyta in this region has been scanty. In order to get a thorough understanding of its Charophyta, we identified specimens collected from 13 countries or cities in this province. The result shows that is especially abundant in Charophyta. In this paper, however, only are reported new species, new varieties and new records of China on the Nitelleae. The former two are Nitella hokouensis, N. bicornuta, N. longicaudata, N. procera, N. brevidactyla, N. papillata, N. pseudohyalina, N. hyalina var. aberrans and Tolypella yunnanensis; while the third is Nitella globulifera Pal andN. japonica T. F. Alle