J Syst Evol ›› 2010, Vol. 48 ›› Issue (4): 257-264.doi: 10.1111/j.1759-6831.2010.00084.x

• Research Articles • Previous Articles     Next Articles

Effect of sampling strategy on estimation of fine-scale spatial genetic structure in Androsace tapete (Primulaceae), an alpine plant endemic to Qinghai-Tibetan Plateau

1Li-Yan ZENG 1Ling-Li XU 2Shao-Qing TANG 3Tashi TERSING 4Yu-Peng GENG* 3Yang ZHONG*   

  1. 1(Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China)
    2(School of Life Sciences, Guangxi Normal University, Guilin 541004, China)
    3(Institute of Biodiversity Science and Geobiology, Tibet University, Lhasa 850000, China)
    4(Institute of Ecology and Geobotany, School of Life Sciences, Yunnan University, Kunming 650091, China)
  • Received:2010-03-06 Online:2010-05-16 Published:2010-07-12

Abstract: The fine-scale spatial genetic structure (SGS) of alpine plants has received increasing attention, from which the seed and pollen dispersal can be inferred. However, estimation of SGS may depend strongly on the sampling strategy, including the sample size and spatial sampling scheme. Here, we examined the effects of sample size and three spatial schemes (i.e., simple-random, line-transect, and random-cluster sampling) on the estimation of SGS in Androsace tapete, an alpine cushion plant endemic to Qinghai-Tibetan Plateau. Using both real data and simulated data of dominant molecular markers, we show that: 1) SGS is highly sensitive to sample strategy especially when the sample size is small (e.g., below 100); 2) the common used SGS parameter (i.e., the intercept of autocorrelogram) is more susceptible to sample error than a new developed Sp statistic; 3) the random-cluster scheme is susceptible to obvious bias in parameter estimation even when the sample size is relatively large (e.g., above 200). Overall, the line-transect scheme is recommendable, which performs slightly better than the simple-random scheme in parameter estimation and is more efficient to encompass broad spatial scales. The consistency between simulated data and real data implies that these findings might hold true in other alpine plants and more species should be examined in future work.

Key words: alpine plant, Androsace tapete, sampling strategy, simulation, spatial genetic structure, spatial autocorrelation.

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