J Syst Evol ›› 2013, Vol. 51 ›› Issue (2): 142-153.doi: 10.1111/j.1759-6831.2012.00226.x

• Research Articles • Previous Articles     Next Articles

Exploring the utility of three nuclear regions to reconstruct reticulate evolution in the fern genus Asplenium

1,2Harald SCHNEIDER* 2Adriana NAVARRO-GOMEZ 2Stephen J. RUSSELL 2Stephen ANSELL 2Michal GRUNDMANN 2Johannes VOGEL   

  1. 1(State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China)
    2(Department of Life Sciences, Natural History Museum, London SW7 5BD, UK)
  • Received:2012-06-11 Online:2012-08-20 Published:2012-10-08

Abstract: Three nuclear regions nuclear ribosomal internal transcribed spacer (nrITS) and intron regions of two nuclear low-copy genes—gapCp, and pgiC, in combination with one chloroplast genome region were employed to explore patterns of reticulate evolution in the fern genus Asplenium. This is the one of the first studies using DNA sequences of multiple nuclear markers in ferns. All three nuclear markers amplified well with PCR and several copies were recovered by cloning PCR products. All three nuclear regions showed congruent results by recovering the neo-allotetraploid Asplenium adulterinum as the hybrid of diploid A. trichomanes and diploid A. viride. Conflicting results were obtained for several nodes. First, gapCp did not discriminate between A. aethiopicum and A. praegracile whereas the other markers recovered these two taxa as distinct. Conflicts among gene-trees were found in respect to A. monanthes, chloroplast and pgiC suggested a sister relationship of A. monanthes and A. trichomanes but gapCp and nrITS nested A. monanthes within A. normale. Our results confirm: (i) the usefulness of several nuclear regions, in particular gapCp