J Syst Evol ›› 2013, Vol. 51 ›› Issue (4): 426-434.doi: 10.1111/j.1759-6831.2012.00243.x

• Research Articles • Previous Articles     Next Articles

Molecular evidence for natural hybridization between Rhododendron spiciferum and R. spinuliferum (Ericaceae)

1,2,3Li-Jun YAN 1Lian-Ming GAO* 1,2De-Zhu LI*   

  1. 1(Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China)
    2(Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China)
    3(University of Chinese Academy of Sciences, Beijing 100049, China)
  • Received:2012-10-29 Online:2012-11-29 Published:2013-03-24

Abstract: Rhododendron×duclouxii is morphologically intermediate between Rhododendron spiciferum and Rhododendron spinuliferum, and was suspected to be a natural hybrid between these species. In this study, the nuclear ribosomal internal transcribed spacer (ITS) and chloroplast trnL-F regions of 34 accessions of R. ×duclouxii and its putative parents were analyzed for evidence of hybridization. The results from molecular data supported by morphology demonstrated that R. ×duclouxii represents a natural hybrid between R. spiciferum and R. spinuliferum. It also implies that the hybridization between R. spiciferum and R. spinuliferum is bidirectional, and that populations of R. ×duclouxii represent hybrid swarms from backcrossing or selfing of F1 plants. The study is important for understanding the speciation processes in the genus since it demonstrates for the first time the existence of a natural homoploid hybrid in Rhododendron subgenus Rhododendron.

Key words: homoploid, ITS, natural hybridization, R. ×duclouxii, R. spiciferum, R. spinuliferum, trnL-F.

[1] Luiz Henrique M. Fonseca and Lúcia G. Lohmann. Exploring the potential of nuclear and mitochondrial sequencing data generated through genome‐skimming for plant phylogenetics: A case study from a clade of neotropical lianas . J Syst Evol, 2020, 58(1): 18-32.
[2] Jose L. Villar, M. Ángeles Alonso, Ana Juan, John F. Gaskin, and Manuel B. Crespo. Out of the Middle East: New phylogenetic insights in the genus Tamarix (Tamaricaceae) . J Syst Evol, 2019, 57(5): 488-507.
[3] Zhi-Yao Ma, Jun Wen, Jing-Pu Tian, Abbas Jamal, Long-Qing Chen, Xiu-Qun Liu. Testing reticulate evolution of four Vitis species from East Asia using restriction‐site associated DNA sequencing . J Syst Evol, 2018, 56(4): 331-339.
[4] W. John Kress. Plant DNA barcodes: Applications today and in the future . J Syst Evol, 2017, 55(4): 291-307.
[5] Wen-Kui Dai, Edwin Luguba Kadiori, Qing-Feng Wang, Chun-Feng Yang. Pollen limitation, plasticity in floral traits, and mixed mating system in an alpine plant Pedicularis siphonantha (Orobanchaceae) from different altitudes . J Syst Evol, 2017, 55(3): 192-199.
[6] Yi-Xuan Kou, Kun Xiao, Xiao-Rong Lai, Yu-Jin Wang, Zhi-Yong Zhang. Natural hybridization between Torreya jackii and T. grandis (Taxaceae) in southeast China . J Syst Evol, 2017, 55(1): 25-33.
[7] Partha Sarathi Saha, Mainak Sengupta, Sumita Jha. Ribosomal DNA ITS1, 5.8S and ITS2 secondary structure, nuclear DNA content and phytochemical analyses reveal distinctive characteristics of four subclades of Protasparagus . J Syst Evol, 2017, 55(1): 54-70.
[8] Erin M. Sigel. Genetic and genomic aspects of hybridization in ferns . J Syst Evol, 2016, 54(6): 638-655.
[9] Paul M Peterson, Konstantin Romaschenko, Yolanda Herrera Arrieta. Phylogeny and subgeneric classification of Bouteloua with a new species, B. herrera-arrietae (Poaceae: Chloridoideae: Cynodonteae: Boutelouinae) . J Syst Evol, 2015, 53(4): 351-366.
[10] Gemma MAS DE XAXARS, Alfredo GARCíA-FERNÁNDEZ, Pere BARNOLA, Joan MARTíN, Arnau MERCADÉ, Joan VALLÉS, Pablo VARGAS, Josep VIGO, Teresa GARNATJE. Phylogenetic and cytogenetic studies reveal hybrid speciation in Saxifraga subsect. Triplinervium (Saxifragaceae) . J Syst Evol, 2015, 53(1): 53-62.
[11] Zi-Yi CHEN, Zhu-Jun XIONG, Xiao-Yun PAN, Su-Qin SHEN, Yu-Peng GENG, Cheng-Yuan XU, Jia-Kuan CHEN, Wen-Ju ZHANG. Variation of genome size and the ribosomal DNA ITS region of Alternanthera philoxeroides (Amaranthaceae) in Argentina, the USA, and China . J Syst Evol, 2015, 53(1): 82-87.
[12] Szilvia STRANCZINGER, Aniko GALAMBOS, Dalma SZENASY, Balint SZALONTAI. Phylogenetic relationships in the Neotropical tribe Hamelieae (Rubiaceae, Cinchonoideae) and comments on its generic limits . J Syst Evol, 2014, 52(5): 643-650.
[13] Yong-Peng MA, Xiao-Ling TIAN, Jing-Li ZHANG, Zhi-Kun WU, Wei-Bang SUN. Evidence for natural hybridization between Primula beesiana and P. bulleyana, two heterostylous primroses in NW Yunnan, China . J Syst Evol, 2014, 52(4): 500-507.
[14] De-Qing HUANG   Qin-Qin LI   Chun-Jing ZHOU   Song-Dong ZHOU   Xing-Jin HE. Intraspecific differentiation of Allium wallichii (Amaryllidaceae) inferred from chloroplast DNA and internal transcribed spacer fragments . J Syst Evol, 2014, 52(3): 341-354.
[15] Shuo YU, Miao-Miao SHI, Xiao-Yong CHEN. Species diversity and distribution of Ruppia in China: Potential roles of long-distance dispersal and environmental factors . J Syst Evol, 2014, 52(2): 231-239.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Lin Zhong-ping. Isolating of mRNA and Polysome from Plant[J]. Chin Bull Bot, 1984, 2(05): 52 -54 .
[2] Meng Xiao-xiong. [J]. Chin Bull Bot, 1988, 5(02): 99 .
[3] Zhigang Huang;Huang Hu;Xuanzhi Zhao;Ruozhong Wang;Langtao Xiao *. Cloning and Bioinformatics Analysis of TIR1 cDNA from Super Hybrid Rice Parental Line 1S[J]. Chin Bull Bot, 2008, 25(06): 695 -700 .
[4] Mingkun Liu;Guanjun Liu;Xiufeng Yan. Co-expression of Polygonum sibiricum Glutathione Transferase and Cysteine Synthase Genes in Saccharomyces cerevisiae[J]. Chin Bull Bot, 2008, 25(06): 687 -694 .
[5] Dahong Li;Hui Liu;Yanli Yang;Pingping Zhen;Jiansheng Liang. Down-regulation of OsRACK1 by Anti-sense Approach Results in Drought Tolerance Enhancement in Rice (Oryza sativa)[J]. Chin Bull Bot, 2008, 25(06): 648 -655 .
[6] Xiuxia He;Yuanhua Yu*;Yong Zhang. Genetic Transformation of PSY Gene in Ginseng[J]. Chin Bull Bot, 2008, 25(05): 579 -584 .
[7] Juan Shi Baohua Zhu Kehou Pan. Advances in Research into the Production of Very Long-chain Polyunsaturated Fatty Acids in Transgenic Plants[J]. Chin Bull Bot, 2007, 24(05): 659 -666 .
[8] Jiyan Qi Jianghua Yang Chaorong Tang. Sucrose Transporter Genes and Their Functions in Plants[J]. Chin Bull Bot, 2007, 24(04): 532 -543 .
[9] Lu Xue-hua and Chen Yang-chun. Tissue Culture and Plantlet Regeneration from Bulb of Lycoris aurea[J]. Chin Bull Bot, 1989, 6(03): 169 .
[10] NIE Lan-Chun② SUN Jian-She HUANG Rui-Hong. The Biosynthesis and Affecting Factors of Aroma in Some Fruits[J]. Chin Bull Bot, 2004, 21(05): 631 -637 .