J Syst Evol ›› 2014, Vol. 52 ›› Issue (5): 580-588.doi: 10.1111/jse.12099

• Research Articles • Previous Articles     Next Articles

Genetic analysis of Anatolian apples (Malus sp.) by simple sequence repeats

1Masum BURAK 2Ali ERGÜL* 3Kemal KAZAN 4Mehmet Emin AKÇAY 2Canan YÜKSEL 2Melike BAKIR 2Filiz MUTAF 2A. Emre AKPINAR 5A. Semih YASASIN 6Hamit AYANOGLU   

  1. 1(Ministry of Food, Agriculture and Livestock, General Directorate of Agricultural Research and Policy, Ankara 06060, Turkey)
    2(Ankara University, Biotechnology Institute, Ankara 06100, Turkey)
    3(Commonwealth Scientific and Industrial Research Organization (CSIRO) Plant Industry, Queensland Bioscience Precinct, St. Lucia, Queensland 4067, Australia)
    4(Ministry of Food, Agriculture and Livestock, General Directorate of Agricultural Research and Policy, Atatürk Central Horticultural Research Institute, Yalova 77100, Turkey)
    5(Ministry of Food, Agriculture and Livestock, General Directorate of Agricultural Research and Policy, Institute of Viticulture, Tekirdag 59100, Turkey)
    6(The Republic of Turkey, Prime Ministry, Ankara 06573, Turkey)
  • Received:2013-09-17 Online:2014-03-21 Published:2014-09-18

Abstract: The apple is one of the most important fruit species in the world. Turkey has a diverse and ancient apple germplasm that have played a major role in the domestication of the Malus genus. However, so far locally grown Anatolian apple germplasm has largely been uncharacterized. In this study, 171 local apple (M. domestica Borkh.) accessions originated from eco-geographically diverse regions of Anatolia were studied using 16 SSR (simple sequence repeat) loci, which generated 254 alleles. Of the SSR markers used, the CH04g10 locus showed the highest allele diversity. Relatively high genetic similarities were found between some accessions. The factorial correspondence analysis did not clearly separate different all apple accession groups, suggesting that Anatolian apple accessions are highly intermixed. However, most apple accessions were grouped according to their collection sites in structure analyses. In addition, reflecting the richness of the Anatolian apple germplasm, low numbers of synonymous, and identical accessions were identified among the germplasm. Finally, using the publically available SSR data generated in other studies, we investigated genetic relationships between Anatolian accession groups and European apple accession groups. Our results reported here provide a useful base for future studies aimed at investigating the genetic diversity of wild and cultivated apples from Anatolia and the surrounding regions.

Key words: Anatolia, apple (Malus domestica Borkh.), genetic resources, microsatellites, Turkey.

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