J Syst Evol ›› 2015, Vol. 53 ›› Issue (2): 166-178.doi: 10.1111/jse.12142

• Research Articles • Previous Articles     Next Articles

Distyly and cryptic heteromorphic self-incompatibility in Mussaenda macrophylla (Rubiaceae)

Xiao-Qin WU1, Pei-Xing LI1,3, Xiao-Fang DENG1,2, and Dian-Xiang ZHANG1*   

  1. 1Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China
    2State Academy of Forestry Administration, Beijing, China
    3University of Chinese Academy of Sciences, Beijing, China
  • Received:2014-01-13 Online:2014-12-25 Published:2015-03-05

Abstract: Typical distylous species display both reciprocal herkogamy and heteromorphic incompatibility, which exclude self- and intramorph pollination and accordingly promote intermorph pollination. Here we explore an unusual distylous flower associated with self- and intramorph compatibility in Mussaenda macrophylla Wall. The species did not exhibit a precise reciprocal herkogamy, and the populations studied had a slight bias in favor of the short-styled morph (S-morph). In addition, pollen tube growth in intermorph pollination was faster than either in intramorph or self-pollination, indicating that cryptic heteromorphic self-incompatibility occurred in M. macrophylla. Specifically, pollen tube growth rates after legitimate and illegitimate pollination were much more highly differentiated in long-styled morph (L-morph) than in S-morph, which might be attributed to the increased de-esterified pectin in the tip of L-morph pollen tube wall in comparison with S-morph. Transmission electron microscopic observation revealed illegitimate self-pollinated pollen tubes of two morphs were seriously degraded with the large autolysosome-like vacuoles in cytoplasm of L-morph pollen tube but accumulating small vacuoles in that of S-morph pollen tube, suggesting that incompatible pollen tubes might undergo autophagic programmed cell death except self-incompatible S-morph pollen tubes. Our results indicate that M. macrophylla is morphologically distylous with a cryptic heteromorphic self-incompatibility breeding system, which functions differently in S-morph with self-pollination compared with other illegitimate pollination.

Key words: cryptic self-incompatibility, distyly, Mussaenda macrophylla, pollen tube growth, Rubiaceae

[1] Szilvia STRANCZINGER, Aniko GALAMBOS, Dalma SZENASY, Balint SZALONTAI. Phylogenetic relationships in the Neotropical tribe Hamelieae (Rubiaceae, Cinchonoideae) and comments on its generic limits . J Syst Evol, 2014, 52(5): 643-650.
[2] Shi CHEN,Zhong-Lai LUO, Dian-Xiang ZHANG. Self-pollination in buds and homostyly in Mussaenda shikokiana (Rubiaceae), a monomorphic species in a distylous clade . J Syst Evol, 2013, 51(6): 731-742.
[3] Leandro FREITAS, Mariana ANDRICH. Is the pollination biology of Bathysa and Schizocalyx consistent with their segregation? A first approach for two sympatric species in southeastern Brazil . J Syst Evol, 2013, 51(5): 522-535.
[4] Hai-Zhen WEN, Rui-Jiang WANG. Foonchewia guangdongensis gen. et sp. nov. (Rubioideae: Rubiaceae) and its systematic position inferred from chloroplast sequences and morphology . J Syst Evol, 2012, 50(5): 467-476.
[5] Xing GUO,Mark P. SIMMONS,Paul Pui-Hay BUT,Pang-Chui SHAW, Rui-Jiang WANG. Application of DNA barcodes in Hedyotis L. (Spermacoceae, Rubiaceae) . J Syst Evol, 2011, 49(3): 203-212.
[6] Min CAI, Hua ZHU, Hong WANG. Pollen morphology of the genus Lasianthus (Rubiaceae) and related taxa from Asia . J Syst Evol, 2008, 46(1): 60-70.
[7] DUAN Lin-Dong, LIN Qi. Taxonomic notes on some species of Ophiorrhiza (Rubiaceae) from China . J Syst Evol, 2007, 45(6): 870-879.
[8] WANG Rui-Jiang. Hedyotis koiana R. J. Wang, a new species of Rubiaceae from China . J Syst Evol, 2007, 45(5): 696-700.
[9] WEN Xiang-Ying, LIN Qi. Validation of names of Leptodermis pilosa var. acanthoclada and L. pilosa var. spicatiformis (Rubiaceae) . J Syst Evol, 2007, 45(3): 410-412.
[10] ZHANG Mei-De, CHEN Wen-Hong, SHUI Yu-Min. Miscellaneous notes on the tribe Gardenieae (Rubiaceae) from China and Vietnam . J Syst Evol, 2007, 45(1): 90-93.
[11]

LI Heng, DAO Zhi-Ling, LI Rong. Reappraisal of Fosbergia shweliensis (Rubiaceae), a species endemic to the Gaoligong Mountains, Western Yunnan, China . J Syst Evol, 2006, 44(6): 707-711.

[12] DENG Xiao-Fang, ZHANG Dian-Xiang. Three new synonyms in Mussaenda (Rubiaceae) from China . J Syst Evol, 2006, 44(5): 608-611.
[13] WANG Rui-Jiang, XING Fu-Wu. Two new species of the genus Hedyotis (Rubiaceae) from China . J Syst Evol, 2003, 41(1): 85-88.
[14] FANG Ding, XIE Zhi-Ming. Three new species of the Rubiaceae from Guangxi, China . J Syst Evol, 2002, 40(2): 154-158.
[15] ZHU Hua. A taxonomic revision of the genus Lasianthus Jack. (Rubiaceae) from Thailand . J Syst Evol, 2001, 39(2): 116-150.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] CHENG Wang-Da ZHAO Guo-PingYao Hai-GenZHANG Guo-Ping. Application of Canopy Temperature in Screening ofRice Genotypes with Resistance to Drought[J]. Chin Bull Bot, 2001, 18(01): 70 -75 .
[2] LIU Jie MO Jie-Sheng LIU Gong-She QI Dong-Mei LI Fang-Fang. Advances in Study on Sunflower Molecular Biology[J]. Chin Bull Bot, 2001, 18(01): 31 -39 .
[3] Xu Bing-sheng and Gu De-xing. Polymorphism in plant population[J]. Chin Bull Bot, 1983, 1(01): 24 -25 .
[4] ZENG Xian-Dong YU Long-Jiang LI Wei YANG Ying WU Yun. Stability of Carbonic Anhydrase in Leaves of Vitex negundo in Karst Areas of Southwest China[J]. Chin Bull Bot, 2005, 22(02): 169 -174 .
[5] Zhou Chang. Advances in Research on Fusion of Plant Reproductive Cell[J]. Chin Bull Bot, 1994, 11(04): 12 -16 .
[6] Cai De-tian and Zhou Chang. Induction of Haploid Plantlets by Anther Culture in Daucus carrota L.[J]. Chin Bull Bot, 1983, 1(02): 36 -37 .
[7] Yu Tetsun. Book Review: The Bibliography of Chinese Botany. 1857-1981[J]. Chin Bull Bot, 1985, 3(02): 64 .
[8] Ke Shan-qiang;Gui Yao-lin and Guo Zhong-chen. Studies on the Artificial Seeds of Plant[J]. Chin Bull Bot, 1989, 6(04): 205 -210 .
[9] Zhang Zhen-jue. Some Principles Governing Shedding of Flowers and Fruits in Vanilla fragrans[J]. Chin Bull Bot, 1985, 3(05): 36 -37 .
[10] Jiang Yue-ming;Lin Shu-xian and Chen Fang. The Granulation and Control of Harvested Citrus Fruit[J]. Chin Bull Bot, 1991, 8(02): 9 -12 .