J Syst Evol ›› 2017, Vol. 55 ›› Issue (1): 16-24.doi: 10.1111/jse.12207

• Research Articles • Previous Articles     Next Articles

DNA barcoding of East Asian Amentotaxus (Taxaceae): Potential new species and implications for conservation

Lian-Ming Gao1*, Yan Li2, Loc Ke Phan3, Li-Jun Yan1,4,5, Philip Thomas6, Long Ke Phan7, Michael Möller6, and De-Zhu Li1,4,5   

  1. 1Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China
    2Institute of Alpine Economic Plants, Yunnan Academy of Agricultural Sciences, Lijiang 674100, Yunnan, China
    3Faculty of Biology, Vietnam National University − University of Science, Hanoi, Vietnam
    4Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China
    5University of the Chinese Academy of Sciences, Beijing 100049, China
    6Royal Botanic Garden Edinburgh, Edinburgh EH3 5LR, UK
    7Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, Hanoi, Vietnam
  • Received:2016-02-28 Online:2016-04-18 Published:2017-01-12

Abstract: DNA barcoding is a useful tool for species identification using standardized genomic DNA fragments. The genus Amentotaxus, consisting of five or six species, is confined to South China, Northeast India, Laos, and Vietnam. All species have been assessed as globally or nationally threatened. However, there is uncertainty about the number of species involved, especially in the border areas of southern China, northern Vietnam, and Laos. We selected five DNA barcodes (rbcL, matK,trnH-psbA, trnL-F, and internal transcribed spacer (ITS)) to evaluate their discrimination ability in this genus, and to investigate the current taxonomy of Amentotaxus. Our results indicate that all the selected barcoding regions showed a high level of universality for PCR and sequencing. When six species are recognized, the nuclear ribosomal DNA region ITS and the chloroplast DNA region trnL-F used on their own provided the highest identification success (60%). Two barcode combinations that included either ITS or trnL-F had the same species discrimination ability. Combinations using additional barcodes did not improve the species identification success. When only five species are recognized, with A. hatuyenensis T. H. Nguyen treated as a synonym of A. yunnanensis H. L. Li, the discrimination rate rises to 100%. Our results also indicate that recent collections from Yunnan province, China, Lao Cai province, Vietnam, and Laos may represent a potential new species. The findings from this study will be very useful for the formulation of appropriate conservation strategies for threatened Amentotaxus species in national and trans-boundary regions.

Key words: Amentotaxus, biodiversity conservation, DNA barcoding, new species discovery, species discrimination

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