J Syst Evol ›› 2008, Vol. 46 ›› Issue (3): 391-395.doi: 10.3724/SP.J.1002.2008.08023

• Research Articles • Previous Articles     Next Articles

Molecular evidence for natural hybridization between Sonneratia alba and S. griffithii

1Suo QIU, 1Ren-Chao ZHOU, 2Yun-Qin LI, 3Sonjai HAVANOND, 3Chanop JAENGJAI, 1Su-Hua SHI*


  1. 1(State Key Laboratory of Biocontrol and Key Laboratory of Gene Engineering of the Ministry of Education, Sun Yat-Sen University, Guangzhou 510275, China)

    2(Experimental Center of Fundamental Teaching, Zhuhai Campus, Sun Yat-Sen University, Zhuhai 519802, China)

    3(Department of Marine and Coastal Resources, Phayathai, Bangkok 10400, Thailand)
  • Received:2008-02-20 Online:2008-03-17 Published:2008-05-18

Abstract: Interspecific hybridization has been frequently observed in the mangrove genus Sonneratia. However, no natural hybridization has been reported between Sonneratia alba and S. griffithii to date, despite their overlapping distribution in the coast of Andaman Sea. In this study, cysteine proteinase inhibitor gene (cpi) from the nuclear genome, and two intergenic spacers (trnL-trnF and trnV-trnM) from the chloroplast genome, were sequenced to determine whether natural hybridization took place between the two species. Our results revealed two distinct types of cpi sequences from the putative hybrid matching those acquired from S. griffithii and S. alba, respectively. Sequencing of the chloroplast trnL-trnF and trnV-trnM regions showed that S. alba differed from S. griffithii by one nucleotide in each region, and the putative hybrid had the identical sequences with S. griffithii. Molecular data demonstrated clearly that there indeed existed natural hybridization between S. alba and S. grif-fithii, and that S. griffithii was the maternal parent in this hybridization event.

Key words: chloroplast DNA, mangroves, natural hybridization, nuclear gene, Sonneratia

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[1] Qifeng Wang, Qiong Yi, Kunzhi Li, Limei Chen, Yongxiong Yu. Construction of a Suppression Subtractive Hybridization Library for Stylosanthes guianensis Under Aluminum Stress and Expressed Sequence Tag Analysis[J]. Chin Bull Bot, 2010, 45(06): 679 -688 .
[2] Zhou Zhi-qin. A Study on the Application of the Median Elimination Series[J]. Chin Bull Bot, 1995, 12(03): 54 -58 .
[3] Luo Jian-Ping and Li Yuan. Conditioning Factors Influencing Plant Single Cell Clone[J]. Chin Bull Bot, 1995, 12(增刊): 17 -20 .
[4] Jia Shi-rong and Cao Dong-sun. Transgenic Plants[J]. Chin Bull Bot, 1992, 9(02): 3 -15 .
[5] ;MA Xiao-Di PENG Hui-Ru WANG Mao WANG Li② SUN Qi-Xin. Evaluation of Heat Tolerance in Crop[J]. Chin Bull Bot, 2004, 21(04): 411 -418 .
[6] Zhao Gao-fan. Callas Induction and Organ Regeneration in Tissue of Fritillaria ussuriensis Maxim[J]. Chin Bull Bot, 1983, 1(02): 40 -41 .
[7] Jian Ling-cheng Sun Long-hua Wei Xiang-yun Wang Hong Zhang Hong. From the studies on the stability of Cellular Membrane System in Relation with Plant Cold Hardiness to the Creation of Plant Cold-resister[J]. Chin Bull Bot, 1994, 11(特辑): 1 -22 .
[8] Wenjie Wang;Yu Guan;Yuangang Zu*;Wenbo Liao;Zhonghua Zhang;Huafeng Chen;Haisheng He. Light Reflectance, Transmittance, and Utilization Efficiency of Leaves of Mikania micrantha and Interspecies Comparison within the Same Community[J]. Chin Bull Bot, 2008, 25(06): 638 -647 .
[9] Hui Huang;Xiaocheng Jiang;Hongyan Cheng;Songquan Song. Progress of Study of Seed Proteomes[J]. Chin Bull Bot, 2008, 25(05): 597 -607 .
[10] Yongshu Liang;Yanping Li;Haibo Sun;Meizhi Zou;Jingyu Wang;Xuejun Liu;Ping Li* . Analysis of Major Agronomic Traits of F2, F3 and F6 Progenies Derived from a Rice Subspecies Cross Pei’ai 64s/Nipponbare[J]. Chin Bull Bot, 2008, 25(01): 59 -66 .