J Syst Evol ›› 2017, Vol. 55 ›› Issue (5): 437-445.doi: 10.1111/jse.12245

• Research Articles • Previous Articles     Next Articles

Insights into the plastid diversity of Daphne blagayana Freyer (Thymelaeaceae)

Živa Fišer Pečnikar1, Nataša Fujs1, Robert Brus2, Dalibor Ballian3,4, and Elena Buzan1*   

  1. 1Department of Biodiversity, Faculty of Mathematics, Natural Sciences and Information Technologies, University of Primorska, Glagoljaška 8, 6000 Koper, Slovenia
    2Department of Forestry and Renewable Forest Resources, Biotechnical Faculty, University of Ljubljana, Večna pot 83, 1000 Ljubljana, Slovenia
    3Faculty of Forestry, University of Sarajevo, Zagrebačka 20, 71000 Sarajevo, Bosnia and Herzegovina
    4Slovenian Forestry Institute, Večna pot 2, 1000 Ljubljana, Slovenia
  • Received:2016-07-26 Online:2017-02-02 Published:2017-09-20

Abstract: Daphne blagayana is a small decumbent bush from the family Thymelaeaceae with a fragmented distribution range, comprising the Balkan Peninsula and the southern Romanian Carpathians. Due to its rarity it is protected by national legislation in almost all countries within its range. The aim of this work was to assess the plastid diversity of Daphne blagayana from 21 locations throughout its distribution range using the chloroplast intergenic regions psbA-trnH and trnK–matK with the gene matK. Moreover, we tried to determine with DNA and morphometric characters whether the division of the species into subspecies and varieties, which were mentioned in some literature (subsp. lerchenfeldiana, var. kellereri, subsp. zogovićii), reflects its phylogeographic structure. The results suggest the existence of three clusters: the northwestern cluster (ESU1), including Slovenian and Italian populations, the southern cluster (ESU2), including populations from Macedonia, Montenegro and some populations from southern Bosnia and Herzegovina, and the central cluster, including the remaining populations (Bulgaria, Romania and rest of the populations from Bosnia and Herzegovina). The results of the genetic as well as morphometric study do not support the existence of subsp. lerchenfeldianaand var. kellereri as distinct infraspecific categories of D. blagayana, thus we suggest that ESU's should be used instead in planning conservation actions.

Key words: Balkan Peninsula, conservation genetics, cpDNA, endemic plants, genetic structure, refugia

[1] Shan-Shan Li, Hai-Fei Zhou, Wen-Li Chen, Juan Yan, Zhe Cai, Ruo-Xun Wei, Chih-Hui Chen, Bin Han, Jian-Qiang Li, Tao Sang and Song Ge. Population genetics and evolutionary history of Miscanthus species in China . J Syst Evol, 2019, 57(5): 530-542.
[2] Soo-Rang Lee, John F. Gaskin, and Young-Dong Kim. Molecular diagnosis for a Tamarix species from two reclaimed lands along the Yellow Sea in Korea inferred from genome wide SNP markers . J Syst Evol, 2019, 57(3): 247-255.
[3] Chuan Chen, Li Zheng, Qing Ma, Wen-Bin Zhou, Yin Lu, Yun-Peng Zhao, and Cheng-Xin Fu. Impacts of domestication on population genetics of a traditional Chinese medicinal herb, Atractylodes macrocephala (Asteraceae) . J Syst Evol, 2019, 57(3): 222-233.
[4] Hannah Graves, A. Lane Rayburn, Sumin Kim,D. K. Lee. Chloroplast DNA variation within prairie cordgrass (Spartina pectinata Link) populations in the U.S. . J Syst Evol, 2016, 54(2): 104-112.
[5] Hong Qian, Jian Zhang. Are phylogenies derived from family-level supertrees robust for studies on macroecological patterns along environmental gradients? . J Syst Evol, 2016, 54(1): 29-36.
[6] Daniel Vitales, Alfredo García-Fernández, Teresa Garnatje, Jaume Pellicer, Joan Vallès. Phylogeographic insights of the lowland species Cheirolophus sempervirens in the southwestern Iberian Peninsula . J Syst Evol, 2016, 54(1): 65-74.
[7] Yi-Jun Lu, Chuan Chen, Rui-Hong Wang, Ashley N. Egan, Cheng-Xin Fu. Effects of domestication on genetic diversity in Chimonanthus praecox: Evidence from chloroplast DNA and amplified fragment length polymorphism data . J Syst Evol, 2015, 53(3): 239-251.
[8] Prabodh K. BAJPAI, Ashish R. WARGHAT, Anil KANT, Ravi B. SRIVASTAVA, Tsering STOBDAN. Detecting molecular signatures of natural selection in Morus alba populations from trans-Himalaya . J Syst Evol, 2014, 52(5): 589-597.
[9] Xi LU, Li CHEN, Ya-Ping CHEN,Hua PENG. Molecular phylogeography and conservation genetics of Sladenia celastrifolia inferred from chloroplast DNA sequence variation . J Syst Evol, 2014, 52(4): 458-465.
[10] Xin-Hui LI, Xin-Xin ZHU, Yang NIU, Hang SUN. Phylogenetic clustering and overdispersion for alpine plants along elevational gradient in the Hengduan Mountains Region, southwest China . J Syst Evol, 2014, 52(3): 280-288.
[11] Liang-Liang YUE, Hang SUN. Montane refugia isolation and plateau population expansion: Phylogeography of Sino-Himalayan endemic Spenceria ramalana (Rosaceae) . J Syst Evol, 2014, 52(3): 326-340.
[12] Yi-Ying LIAO, You-Hao GUO, Jin-Ming CHEN, Qing-Feng WANG. Phylogeography of the widespread plant Ailanthus altissima (Simaroubaceae) in China indicated by three chloroplast DNA regions . J Syst Evol, 2014, 52(2): 175-185.
[13] Shuo YU, Miao-Miao SHI, Xiao-Yong CHEN. Species diversity and distribution of Ruppia in China: Potential roles of long-distance dispersal and environmental factors . J Syst Evol, 2014, 52(2): 231-239.
[14] You-Hua CHEN. A phylogenetic subclade analysis of range sizes of endemic woody seed plant species of China: Trait conservatism, diversification rates and evolutionary models . J Syst Evol, 2013, 51(5): 590-600.
[15] Xue-Mei Zhang, Xing-Jin He. Phylogeography of Angelica nitida (Apiaceae) endemic to the Qinghai–Tibet Plateau based on chloroplast DNA sequences . J Syst Evol, 2013, 51(5): 564-577.
Full text



[1] . [J]. Chin Bull Bot, 1999, 16(增刊): 65 .
[2] Xie Jian-guo;Yan Nai-you;Xuan Hai-bo and Zhang Jie. The Effects of Growth Rctardants on Young Trees of Actinidia chinensis[J]. Chin Bull Bot, 1988, 5(04): 227 -231 .
[3] Du Ling-ge and Hou Yan-hua. Anther Culture of Fritillaria ussuriensis Maxim and Induction of Pollen Plantlets[J]. Chin Bull Bot, 1984, 2(05): 44 -46 .
[4] Li Yun-ying;Cao Min;Dang Feng-liang;Wang Jian;Zhou Yu-shan and Gao Huan. Effect of Different Storage Temperature on the Occurrence of[J]. Chin Bull Bot, 1984, 2(05): 37 -38 .
[5] Li Xian-zhang and Tang Ding-tai. Effect of the Different Preservative Solutions on the Cut Flower Life and Storage of Tulip[J]. Chin Bull Bot, 1995, 12(增刊): 66 -68 .
[6] Lin Zhong-ping and Cui Qing-ling. A Simple Method for Preparing Large Liposome[J]. Chin Bull Bot, 1985, 3(02): 44 .
[7] Liu Wu-lin. Collection,Storeage of Pollen and Dertermination of its Vitality[J]. Chin Bull Bot, 1985, 3(03): 8 -12 .
[8] Lu Tie-gang and Zheng Guo-chang. Current Advances in Biochemical and Physiological Researchs and Molecular Mechanism of Somatic Embryogenesis[J]. Chin Bull Bot, 1989, 6(04): 197 -204 .
[9] Wenxia Wang;Shuguang Li;Xiaoming Zhao;Bingcheng Lin;Yuguang Du. Effects of Oligochitosan on Transcription of Genes Involved in Jasmonic Acid Biosynthesis in Tobacco Suspension Cells[J]. Chin Bull Bot, 2008, 25(05): 526 -532 .
[10] Juan Du;Yourong Chai. Structural Features and Action Mechanisms of Plant Transcriptional Repressors[J]. Chin Bull Bot, 2008, 25(03): 344 -353 .