J Syst Evol ›› 2011, Vol. 49 ›› Issue (1): 65-71.DOI: 10.1111/j.1759-6831.2010.00107.x

• Research Articles • Previous Articles     Next Articles

Phylogenetic relationships among Hystrix species and related species based on expressed sequence tag-polymerase chain reaction

1,2Rui‐Wu YANG* 2Hisashi TSUJIMOTO 1Chun‐Bang DING 1Li ZHANG 1Xiao‐Li WANG 3Yong‐Hong ZHOU   

  1. 1(College of Biology and Science, Sichuan Agricultural University, Yaan 625014, Sichuan, China)
    2(Laboratory of Plant Genetics and Breeding Science, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan)
    3(Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611830, Sichuan, China)
  • Received:2010-04-28 Published:2011-01-19

Abstract: Twelve species, including three Hystrix species, five Leymus species, Hordeum bogdanii, Pseudoroegneria spicata, Psathyrostachys huashanica, and Roegneria ciliaris, were used for expressed sequence tag-polymerase chain reaction (EST-PCR) assay. A total of 125 products were amplified by 72 sets of EST-PCR markers developed in barley, among which 106 (84.8%) products were found to be polymorphic. Each EST-PCR marker produced 0–6 polymorphic bands, with an average of 1.47. The relationship between H. duthiei ssp. duthiei and H. duthiei ssp. longearistata is close, but they are remote to H. patula. Hystrix duthiei ssp. duthiei and H. duthiei ssp. longearistata were clustered with Leymus species and Psathyrostachys huashanica, which suggested that they have close genetic relationships. The results of EST-PCR analysis are basically comparable with those obtained from studies on cytology, which indicated that EST-PCR can be used to assess the genetic relationships among the perennial species in Triticeae.

Key words: cluster analysis, expressed sequence tag, Hystrix, molecular marker, phylogenetic relationship.