J Syst Evol ›› 2011, Vol. 49 ›› Issue (3): 261-266.DOI: 10.1111/j.1759-6831.2011.00133.x

• Research Articles • Previous Articles     Next Articles

Testing the potential of proposed DNA barcodes for species identification of Zingiberaceae

1Lin-Chun SHI 1Jin ZHANG 1Jian-Ping HAN 1Jing-Yuan SONG 1Hui YAO 1Ying-Jie ZHU 3Jia-Chun LI 3Zhen-Zhong WANG 3Wei XIAO* 1Yu-Lin LIN 1Cai-Xiang XIE 2Zhong-Zhi QIAN 1Shi-Lin CHEN*   

  1. 1 (Institute of Medicinal Plant Development, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100193,China)
    2 (China Pharmacopoeia Commission, Beijing 100061,China)
    3 (State Key Laboratory of Pharmaceutical New-tech for Chinese Medicine, Jiangsu Kanion Pharmaceutical Co., Ltd, Lianyungang 222001, China)
  • Received:2010-12-15 Published:2011-06-01

Abstract: In 2009, the Consortium for the Barcode of Life (CBOL) recommended the combination of rbcL and matK as the plant barcode based on assessments of recoverability, sequencing quality, and levels of species discrimination. Subsequently, based on a study of more than 6600 samples belonging to 193 families from seven phyla, the internal transcribed spacer (ITS) 2 locus was proposed as a universal barcode sequence for all major plant taxa used in traditional herbal medicine. Neither of these two studies was based on a detailed analysis of a particular family. Here, Zingiberaceae plants, including many closely related species, were used to compare the genetic divergence and species identification efficiency of ITS2, rbcL, matK, psbKpsbI, trnHpsbA, and rpoB. The results indicate that ITS2 has the highest interspecific divergence and significant differences between inter- and intraspecific divergence, whereas matK and rbcL have much lower divergence values. Among 260 species belonging to 30 genera in Zingiberaceae, the discrimination ability of the ITS2 locus was 99.5% at the genus level and 73.1% at the species level. Thus, we propose that ITS2 is the preferred DNA barcode sequence for identifying Zingiberaceae plants.

Key words: DNA barcoding, identification efficiency, ITS2, Zingiberaceae.