Abstract: Linguistics and genetics always reach similar results in phylogenetic studies of human populations. A previous study found that populations speaking Han Chinese dialects have closer genetic relationships to each other than to neighboring ethnic groups. However, the Pinghua Chinese population from Guangxi is an exception. We have reported that northern Pinghua people are genetically related to populations speaking Daic languages. In this study, we further studied the southern Pinghua population. The Y chromosome and mitochondrial DNA haplogroup components and network analysis indicated that northern and southern Pinghua populations were genetically different. Therefore, we concluded that the Pinghua speakers may have various origins, even though Pinghua dialects are similar. Pinghua dialects might have originated when the Daic or Hmongic speakers from different regions learnt to speak the same Chinese dialect hundreds of years ago. Speakers of one language do not always have just one origin.

Key words: mitochondrial DNA, multiple origins, Pinghua Chinese, Y chromosome.