J Syst Evol ›› 2020, Vol. 58 ›› Issue (2): 103-117.DOI: 10.1111/jse.12526

• Research Articles •     Next Articles

Revisiting the phylogeny of Dipsacales: New insights from phylogenomic analyses of complete plastomic sequences

Chun‐Lei Xiang1†, Hong‐Jin Dong2,3†, Sven Landrein4, Fei Zhao1,5, Wen‐Bin Yu4, Douglas E. Soltis6, Pamela S. Soltis6, Anders Backlund7, Hua‐Feng Wang8, De‐Zhu Li1,9*, and Hua Peng1*   

  1. 1CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
    2Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huanggang 438000, Hubei, China
    3Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang 438000, Hubei, China
    4Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, Yunnan, China
    5University of Chinese Academy of Sciences, Beijing 100049, China
    6Florida Museum of Natural History, University of Florida, Gainesville, Florida 32611, USA
    7Division of Pharmacognosy, Department of Medicinal Chemistry, Uppsala University, Uppsala 75123, Sweden
    8Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Life and Pharmaceutical Sciences, Hainan University, Haikou 570228, China
    9Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Received:2018-11-13 Accepted:2019-06-06 Online:2019-06-27 Published:2020-03-01

Abstract:

Phylogenetic relationships in Dipsacales have long been a major challenge. Although considerable progress has been made during the past two decades, questions remain; the uncertain systematic positions of Heptacodium, Triplostegia, and Zabelia, in particular, impede our understanding of Dipsacales evolution. Here we use 75 complete plastomic sequences to reconstruct the phylogeny of Dipsacales, of which 28 were newly generated. Two primary clades were recovered that form the phylogenetic backbone of Dipsacales. Seven of the primary clades correspond to the recognized families Adoxaceae, Caprifoliaceae s. str., Diervillaceae, Dipsacaceae, Linnaeaceae, Morinaceae, and Valerianaceae, and one corresponds to Zabelia, which was found to be the closest relative of Morinaceae in all analyses. Additionally, our results, with greatly increased confidence in most branches, show that Heptacodium and Triplostegia are members of Caprifoliaceae s. str. and Dipsacaceae, respectively. The results of our study indicate that the complete plastomic sequences provide a fully‐resolved and well‐supported representation of the phylogenetic relationships within Dipsacales.

Key words: Adoxaceae, Caprifoliaceae, Heptacodium, Morinaceae, Triplostegia, Zabelia