J Syst Evol ›› 2004, Vol. 42 ›› Issue (5): 419-426.

• Research Articles • Previous Articles     Next Articles

Mapping of18-26S rDNA loci in four species of the genus Paris by fluorescence in situ hybridization(FISH)

WANG Li, LI Yun-Fei, TANG Rong-Hua, GU Zhi-Jian   

  • Published:2004-09-10

Abstract: 18-26S rDNA loci were mapped on chromosomes in four species of Par is,and the num-ber and position of rDNA sites in these species were compared f or analysis of the distribution of the sites. All the plants were diploids,and t he genome consisted of five chromosomes,A,B,C,D and E. (1)P. polyphylla var. yunnanensis,2n=10=6m+4t. Two18-26S rDNA loci were de-tected on the short arms o f C and D chromosomes;(2)P. forrestii,2n=10=6m+4t. One locus was detected on th e long arm of B chromosome,and also two loci on the short arms of C and D chromosomes;(3)P. axialis. 2n=10=6m(2sat)+4t(2sat)+1-2B. Two loci were detected o n the short arms of C and D chromosomes. One locus was detected in the cell with t wo B-chromosomes(B),but none was detected in that with only one B chromosome, indicating that rRNA gene existed on B chromsome,and an unequal division occurr ed during mitotic cycle of B-chromosomes. (4)P. daliensis,2n=10=4m+2sm+2st+2t. O ne locus was detected on the short arm of D chromo-some. The signals of18-26S rD NA appeared not only in the second constriction but also in the other regions of chromosome. It is noteworthy that one locus was detected in the terminal region o n the short arm of C chromosome in all the four species studied.

Key words: Paris, fluorescence in situ hybridization(FISH), 18-26S rDNA, B c