J Syst Evol ›› 2016, Vol. 54 ›› Issue (5): 528-534.DOI: 10.1111/jse.12205

• Research Articles • Previous Articles     Next Articles

Likely allopatric origins of Adiantum × meishanianum (Pteridaceae) through multiple hybridizations

Hui Shang1†, Ying Wang1,2†, Xiao-Feng Zhu1,3, Guo-Hua Zhao1,4, Fan-Hong Wang5, Jin-Mei Lu5, and Yue-Hong Yan1*   

  1. 1National Conservation Center for Endangered Useful Plants in East China; Shanghai Chenshan Botanical Garden; Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai 201602, China
    2College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China
    3State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China
    4Harbin Normal University, Key Laboratory of Plant Biology, College of Heilongjiang Province, Harbin 150025, China
    5Key Laboratory for Plant Biodiversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Received:2015-11-03 Published:2016-09-08

Abstract: Adiantum × meishanianum F. S. Hsu ex Y. C. Liu & W. L. Chiou was regarded as an endemic species in Meishan Village, Kaohsiung, Taiwan, China and a hybrid between A. malesianum Ghatak (the maternal parent) and a sexually reproducing diploid cryptic species of A. philippense L. (the paternal parent), as revealed by chloroplast and nuclear markers. However, morphological research revealed that A. × meishanianum is also disjunctively distributed in Yunnan and that its paternal parent is possibly A. menglianense Y. Y. Qian. Thus, this study aimed to confirm these findings by using two chloroplast regions and a low-copy nuclear marker in DNA barcoding and phylogenetic analyses, spore measurement, and flow cytometry. Our results indicated that A. × meishanianum in Yunnan is triploid and abortive, the same as A. × meishanianum in Taiwan, and they both originated from the hybridization between the maternal parent of A. malesianum and the paternal parent ofA. menglianense, but not A. philippense. In conclusion, A. × meishanianum probably originated from multiple hybridizations in Taiwan and Yunnan.

Key words: Adiantum , × , meishanianum, A. menglianense, cryptic species, disjunctive distribution, multiple hybrids