J Syst Evol ›› 2016, Vol. 54 ›› Issue (1): 75-82.DOI: 10.1111/jse.12165

• Research Articles • Previous Articles     Next Articles

Generic identity of Camptorrhiza indica (Colchicaceae) based on cytogenetics and molecular phylogenetics

Manoj M. Lekhak1†*, Siddharthan Surveswaran2†, and Shrirang R. Yadav1   

  1. 1Angiosperm Taxonomy Laboratory, Department of Botany, Shivaji University, Kolhapur, India
    2Centre for Ecological Sciences, Indian Institute of Science, Bangalore, India
  • Received:2014-12-31 Published:2015-07-28

Abstract: The tribe Iphigenieae (Colchicaceace, Liliales) includes two genera, viz. Camptorrhiza and Iphigenia, which are distributed in Africa, India, and Australasia. Iphigeniais represented by 12 species, of which six occur in India while Camptorrhiza comprises one species each in Africa (C. strumosa) and India (C. indica). The genusCamptorrhiza possesses a knee-shaped tuber attached to the corms, filaments with a thick bulge in the middle and styles with single stigma. Iphigenia on the other hand lacks knee-shaped tuber, bears linear filaments and has styles with three stigmas. Camptorrhiza indica possesses ovoid corms, linear filaments and styles with a single stigma. These characters are intermediate between Iphigenia and Camptorrhiza and hence we studied the cytogenetics and phylogenetic placement of this species to ascertain its generic identity. Somatic chromosome count (2n = 22) and karyotypic features of C. indica are very similar to that of Iphigenia species. Molecular phylogenetic studies based on atpB-rbcL, rps16, trnL, and trnL-F regions showed that C. indica is nested within a lineage of Indian Iphigenia species. Thus, C. indica was reduced to a species of Iphigenia, i.e., I. ratnagirica. Camptorrhiza is now a monotypic genus restricted only to southern Africa. A key to the IndianIphigenia species is provided. In addition, a new combination Wurmbea novae-zelandiae is proposed for Iphigenia novae-zelandiae.

Key words: Camptorrhiza, Iphigenia ratnagirica, karyotype, molecular phylogeny, Wurmbea novae-zelandiae