J Syst Evol ›› 2006, Vol. 44 ›› Issue (3): 296-303.DOI: 10.1360/aps040100

• Research Articles • Previous Articles     Next Articles

Relationship of Asplenium yunnanense and A. lushanense inferred from the sequence analysis of chloroplast   rbcL, trnL-F and rps4-trnS

1LI Chun‐Xiang,2LU Shu‐Gang   

  1. 1(State Key Laboratory of Palaeobiology and Stratigraphy of Nanjing Institute of Geology and Palaeontology,  the Chinese Academy of Sciences, Nanjing 210008, China)

    2(Institute of Ecology and Geobotany, Yunnan University, Kunming 650091, China)cxli@nigpas.ac.cn
  • Received:2004-08-23 Published:2006-05-18

Abstract: The relationship between Asplenium yunnanense Franch. and Asplenium lushanense C. Chr. has long been in dispute, and here was evaluated based on the sequence analysis of chloroplast rbcL gene, trnL-F and rps4-trnS intergenic spacers. No sequence difference in the studied chloroplast DNA regions was found between Asplenium lushanense and A. yunnanense, but some nucleotide differences in the corresponding DNA fragments were found among other Asplenium species. So, A. yunnanense and A. lushanense cannot be distinguishable in terms of chloroplast DNA sequences, and the very close relationship between them is further affirmed based on the evidence from molecular data.

Key words: Asplenium yunnanense, Asplenium lushanense, chloroplast DNA sequence, relationship