J Syst Evol ›› 2006, Vol. 44 ›› Issue (5): 488-493.DOI: 10.1360/aps050044

• Research Articles • Previous Articles     Next Articles

Isozyme analysis of reciprocal interspecific hybrid F1 between Cucumis sativus and its wild relative C. hystrix

1 2LUO Xiang-Dong, 1 2DAI Liang-Fang, 1LIU Qiang, 1LOU Qun-Feng, 1QIAN Chun-Tao, 1CHEN Jin-Feng*   

  1. 1 (State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China)

    2 (College of Life Sciences, Jiangxi Normal University, Nanchang 330027, China)
  • Received:2005-03-14 Published:2006-09-07

Abstract: Electrophoresis of aspartate aminotransferase (AAT), malate dehydrogenase (MDH) and esterase (EST) were performed to characterize and compare the reciprocal interspecific hybrid plants between cultivated cucumber (Cucumis sativus cv. Changchunmici, 2n=14, CC) and its wild relative C. hystrix (2n=24, HH), and their parents. The results indicate that the zymograms in the reciprocal F1 were typically expressed by complementary bands from both parents. In addition, four heterodimeric bands (Aat-1-94, Aat-2-104, Mdh-3-102 and Est-5-102) were observed. All the three isozymes investigated could identify the interspecific hybrid plants. The results also reveal the differences of the reciprocal F1s at the number and intensity of the bands in the zymograms of AAT and MDH, which further confirmed the reciprocal differences in the interspecific hybridization between C. hystrix and C. sativus.

Key words: isozyme, cucumber, interspecific hybrid