Abstract: The authors studied the Giemsa C-bands in the chromosomes of Polygonatum odoratum (Mill.) Druce. The results appeared that the Giemsa C-bands of P. odoratum here were quite different from those of P. odoratum (Mill.) Druce. var peuriflorum (Miq.) Ohwi as studied by Tanaka. A detailed discussion has been given on the Cbanding patterns in Polygonatum odoratum, its significance in the taxonomy of thegenus Polygonatum and on the technique of the Giemsa C-bands staining.