J Syst Evol ›› 2020, Vol. 58 ›› Issue (1): 69-76.DOI: 10.1111/jse.12484

• Research Articles • Previous Articles     Next Articles

Molecular diversity of the 5S nuclear ribosomal DNA in Campeiostachys with StHY haplome constitution

Cai-Rong Yang1,2, Bernard R. Baum3, Douglas A. Johnson3, Hai-Qin Zhang2, and Yong-Hong Zhou2*   

  1. 1College of Chemistry and Life Science, Chengdu Normal University, Chengdu 611130, China
    2Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China
    3Faculty of Science, Department of Biology, University of Ottawa, Ottawa K1N 6N5, Canada
  • Received:2018-04-21 Accepted:2019-01-16 Online:2019-03-27 Published:2020-01-01

Abstract: To detect the genomic constitutions and investigate the evolutionary relationships between Campeiostachys Drobov and Elymus L. species, we have cloned and analyzed 271 5S nuclear ribosomal DNA sequences from 27 accessions of these species, mostly of Chinese origin. We identified Long H1, Short S1, and Long Y1 unit classes in nine Campeiostachys or Elymus species. The identification of the three orthologous unit classes was confirmed by the neighbor‐joining tree of each unit class from PAUP and the phylogeny tree of three unit classes from MrBayes. The results suggested that these Elymus species comprise StYH haplomes and should be included in Campeiostachys. The phylogeny tree showed a clear separation between the S1 unit class and Y1 unit class. However, Y1 unit class sequences formed a sister clade to the S1 unit class, implying that although the St and Y haplomes might have some affinity, they are distinct from one another. The phylogeny tree also indicated that the five species in sect. Turczaninovia (C. dahurica var. cylindrica, C. dahurica var. dahurica, C. dahurica var. tangutorum, E. purpuraristatus, and E. dahuricus Turcz. ex Griseb. var. violeus C. P. Wang & H. L. Yang) might share a more recent common ancestor, whereas the four species in sect. Elymus (C. nutans, E. breviaristatus (Keng) Keng ex Keng f., E. sinosubmuticus (Keng) Keng f., and E. atratus (Nevski) Hand.‐Mazz.) share a close relationship. By identifying only one type of unit class for each haplome, we propose that the 5S nuclear ribosomal DNA sequences of species within Campeiostachys might have undergone haplome‐specific concerted evolution.

Key words: Campeiostachys, Elymus, genome, haplome, unit class