J Syst Evol ›› 2021, Vol. 59 ›› Issue (4): 809-832.DOI: 10.1111/jse.12721

• Research Articles • Previous Articles     Next Articles

Targeted sequencing supports morphology and embryo features in resolving the classification of Cyperaceae tribe Fuireneae s.l.

Julian R. Starr1*, Pedro Jiménez‐Mejías2,3, Alexandre R. Zuntini4, Étienne Léveillé‐Bourret5, Ilias Semmouri6, Muthama Muasya7, William J. Baker4, Grace E. Brewer4, Niroshini Epitawalage4, Isabel Fairlie4,8, Félix Forest4, Izai A. B. Sabino Kikuchi4,9, Lisa Pokorny4, and Isabel Larridon4,10   

  1. 1Department of Biology, University of Ottawa, Ottawa, ON K1N 6N5, Canada
    2Departamento de Biología (Botánica), Facultad de Ciencias Biológicas, Universidad Autónoma de Madrid, C/Darwin, 2, Madrid 28049, Spain
    3Centro de Investigación en Biodiversidad y Cambio Global (CIBC‐UAM), Universidad Autónoma de Madrid, Madrid 28049, Spain
    4Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3AE, UK
    5Institut de recherche en biologie végétale (IRBV), Université de Montréal, Montréal, QC H1X 2B2, Canada
    6Laboratory of Environmental Toxicology and Aquatic Ecology, Faculty of Bioscience Engineering, Ghent University, Ghent 9000, Belgium
    7Department of Biological Sciences, Bolus Herbarium, University of Cape Town, Private Bag X3, Rondebosch, Cape Town 7701, South Africa
    8Department of Animal and Plant Sciences, University of Sheffield, Alfred Denny Building, Western Bank, Sheffield S10 2TN, UK
    9Hortus botanicus Leiden, Universiteit Leiden, PO Box 9500, Leiden 2300 RA, The Netherlands
    10Systematic and Evolutionary Botany Lab, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, Gent 9000, Belgium
    Current address: Lisa Pokorny, Centre for Plant Biotechnology and Genomics (CBGP, UPM‐INIA), 28223 Pozuelo de Alarcón, Madrid, Spain.
  • Received:2020-08-31 Accepted:2020-12-30 Online:2021-01-14 Published:2021-07-01

Abstract: Molecular phylogenetic studies based on Sanger sequences have shown that Cyperaceae tribe Fuireneae s.l. is paraphyletic. However, taxonomic sampling in these studies has been poor, topologies have been inconsistent, and support for the backbone of trees has been weak. Moreover, uncertainty still surrounds the morphological limits of Schoenoplectiella, a genus of mainly small, amphicarpic annuals that was recently segregated from Schoenoplectus. Consequently, despite ample evidence from molecular analyses that Fuireneae s.l. might consist of two to four tribal lineages, no taxonomic changes have yet been made. Here, we use the Angiosperms353 enrichment panel for targeted sequencing to (i) clarify the relationships of Fuireneae s.l. with the related tribes Abildgaardieae, Eleocharideae, and Cypereae; (ii) define the limits of Fuireneae s.s., and (iii) test the monophyly of Fuireneae s.l. genera with emphasis on Schoenoplectus and Schoenoplectiella. Using more than a third of Fuireneae s.l. diversity, our phylogenomic analyses strongly support six genera and four major Fuireneae s.l. clades that we recognize as tribes: Bolboschoeneae stat.nov., Fuireneae s.s., Schoenoplecteae, and Pseudoschoeneae tr. nov. These results are consistent with morphological, micromorphological (nutlet epidermal cell shape), and embryo differences detected for each tribe. At the generic level, most sub-Saharan African perennials currently treated in Schoenoplectus are transferred to Schoenoplectiella. Our targeted sequencing results show that these species are nested in Schoenoplectiella, and their treatment here is consistent with micromorphological and embryo characters shared by all Schoenoplectiella species. Keys to recognized tribes and genera are provided.

Key words:

Angiosperms353, classification,  Cyperaceae,  Fuireneae,  targeted sequencing,  taxon limits, taxonomy