J Syst Evol ›› 2023, Vol. 61 ›› Issue (5): 764-775.DOI: 10.1111/jse.12940

• Research Articles • Previous Articles     Next Articles

RAD sequencing, morphometry and synecology clarify the taxonomy of the Melica ciliata (Poaceae) complex in France and Poland

Sergio Castro1*, Audrey Muratet1, Magdalena Szczepaniak2, Julie Nguefack3, and Laurent Hardion1*   

  1. 1 Laboratoire Image Ville Environnement(LIVE), CNRS, University of Strasbourg, Strasbourg, France;
    2 W. Szafer Institute of Botany, Polish Academy of Sciences, Lubicz 4631-512 Kraków, Poland;
    3 Conservatoire Botanique d'Alsace-Lorraine, Erstein, France
    *Authors for correspondence. Sergio Castro. E-mail:sercastro17@gmail.com;Laurent Hardion. laurent.hardion@live-cnrs.unistra.fr
  • Received:2022-02-27 Accepted:2022-11-23 Online:2022-12-08 Published:2023-09-01

Abstract: Melica (Poaceae) consist of about 92 species distributed across temperate regions of the world. Within section Dalycum, Melica ciliata sensu lato forms a taxonomic complex of several species and subspecies with clinal morphological variation causing conflicting identifications. To resolve taxonomic confusion, we used three complementary approaches, through molecular, morphological, and phytoecological analyses. The double-digest restriction-associated DNA markers significantly support the monophyly of three taxa: (i) the Mediterranean Melica magnolii, (ii) the Eurasian Melica transsilvanica subsp. transsilvanica, and (iii) the west-European M. ciliata subsp. glauca. This differentiation is corroborated by the analysis of 22 morphometric variables. Furthermore, phytoecological analysis of 221 floristic inventories revealed habitat distinctions among these taxa. Our approach of integrative taxonomy argues for a specific distinction for these three taxa, and we include a key to separate these forms. These new molecular data on the section Dalycum, subsection Ciliatae, call for further phylogenetic analyses including samples of M. ciliata subsp. ciliata and other East-Mediterranean and South African taxa.

Key words: double-digest restriction site-associated DNA sequencing (ddRAD), Melica ciliata, morphology, species delimitation, synecology, taxonomy