J Syst Evol ›› 1999, Vol. 37 ›› Issue (2): 125-130.

• Research Articles • Previous Articles     Next Articles

Morphology, fertility and cytogenetics of intergeneric hybrid between Roegneria kamoji Ohwi and Dasypyrum villosum (L.) Candargy (Poaceae: Triticeae)

ZHOU Yong-Hong, WU Bi-Hua, FU Ti-Hua, ZHENG You-Liang   

  • Published:1999-03-10

Abstract: The intergeneric hybrids between Roegneria kamoji Ohwi and Dasypyrum villosum (L.)Candargy were successfully obtained by means of embryo culture in vitro. Studies on morphology, fertility and chromosome pairing behavior in meiosis of the parents and their hybrid Fl were carried out in the present work. The results showed that: (1) there were ob vious morphological differences between R. kamoji and D. villosum, and spikes of Fl plants were morphologically intermediate between the two parental species; (2) the seed set of the cross was 11.63%; the hybrid plant was infertile, which indicated that strong repro ductive isolation existed between the parents and R. kamoji and D. villosum were inde pendent biological species; (3) The somatic chromosome number in root-tips of Fl hybrids was 28. Chromosome pairing at MI of PMCs in Fl hybrids was quite low. The meiotic con figuration was 26.72 Ⅰ + 0.62 Ⅱ + 0.02 Ⅲ, which indicated that very low homoeology was detected between the St, H, Y genomes of R. kamoji and the V genome of D. villo- sum, and the relationship between the parental species was remote.

Key words: Roegneria kamoji, Dasypyrum villosum, Intergeneric hybrid, Meiosis, Genome, Chromosome pairing Relationship