Abstract: Guizhou is situated in the southwestern China and is very rich in algae However, our present knowledge of the Charophytes is scanty. Over 400 specimens were collected from 22 counties or cities and from them 41 species, 2 subspecies, 3 varieties were found. In addition to previously known species of this region, Nitella rasilis, N. paludosa, N. pseudopapillata and N. guizhouensis are new species, N. mirabilis var. libera is new variety, and N. penicillata, Chara burmanica, C. vulgaris var. gymnophylla are new records for China. In this paper, only the new taxa are reported.

Key words: Charophyta, Guizhou, Nitella mirabilis var.libera, N. rasilis, N. paludosa, N. pseudopapillata, N. guizhouensis