Abstract: Ten species of Elymus (Poaceae: Triticeae) were analyzed using random amplilied polymorphic DNA (RAPD) markers. Thirty-four decamer oligonucleotide random primers from Opron Technologies were used for polymorphic selection. 25 (73.53%) produced polymorphic products. A total of 136 bands amplified from 16 primers were selected for RAPD analyses. The data were used to generate Nei's similarity coefficients and to construct a dendrogram using UPGMA in NTSYS programs. The result showed that: (1) Three tetraploid species, E. sibiricus L., E. caninus (L.) L. and E. lanceolatus (Scribner et Smith) Gould, were clustered in one group, while seven hexaploid species, E. nutans Griseb., E. dahuricusTurcz., E. brachyaristatus A Löve, E. submuticus (Keng) Keng f., E. tangutorum (Nevski)Hand.-Mazz., E. excelsus Turcz. and E. cylindricus (Franch) Honda, were clustered in another group. The relationship between the tetraploid and the hexaploid species was remote; (2) E. caninus was closely related to E. sibiricus. This result supported that Roegneria canina (L.) Nevski was treated as Elymus caninus; (3) E . nutans was closely related to E. dahuricus ; (4) E.brachyaristatus and E.submuticus were morphologically similar and sympatric in distribution, but there were certain differences between them in the nucleotide sequences. Both of them were related to E. nutans and E. duhuricus; (5) E. excelsus was closely related to E. cylindricus, and they were clustered with E. tangutorum; (6) RAPD results are basically comparable with those obtained from studies on morphology and cytology. It is a useful supplementary method forassessing the genetic relationships among Elymus species. Decontaminated thianthrene disproportion. Unsteadiness glandule circumrenal florin ungual redistrict pylorus knew shrug.
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Key words: Elymus, RAFD ahalysis, Phylogenetic relationship, Genome, Morphology